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Updated: Aug 9, 2026
Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Published on: July 9, 2008
[Types of chemokines expressed by murine dendritic cell line]
1Department of Pediatrics, People's Hospital, Peking Unviersity, Beijing 100044, China.
Objective:
To analyze the types of chemokines expressed in murine medullary thymic dendritic cell line (MTDC).
Methods:
A MTDC line was established. RT-PCR was used to amplify the cDNA fragments of SDF-1, IP-10, KC, MCP-1, and lymphotactin expressed by MTDC. Nucleotide sequencing and dot blotting analysis were performed to confirm their existence.
Results:
Five cDNA fragments SDF-1, KC, IP-10, MCP-1, and lymphotactin, were amplified from MTDC. The existence of SDF-1, KC, and lymphotactin was confirmed by nucleotide wequencing, and that of IP-10, MCP-1, and lymphotactin was confirmed by dot blotting. The supernatant of MTDC exhibited chemoattractant activities to CD(4)(-) approximately CD(8)(-) thymocytes with a chemotactic index (CI) of 3.4.
Conclusion:
There is a lot of chemokines in MTDC, including C-X-C subfamily (SDF-1, KC, and IP-10) CC subfamily (MCP-1), and C subfamily (lymphotactin). The suparnatant of MTDC has moderately chemotactic activities to CD(4)(-) approximately CD(8)(-) thymocytes, consistent with the biological function of SDF-1.
