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Updated: Aug 18, 2026
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
[Cloning and identifying renal cell carcinoma differentially expressed genes and their significance]
1Department of Urology, The First Hospital, Peking University, Beijing 100034, China.
Objective:
To Clone study the differentially expressed new genes in renal cell carcinoma (RCC).
Methods:
Using a technique known as suppression subtractive hybridization to construct the library which contains the differently expressing cDNAs between RCC and normal kidney cells. Then the RCC specifically expressed genes were cloned.
Results:
Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contained 350 positive clones. Sequence analysis were performed for 5 clones. All the sequences were unknown previously and the cDNA insert GYLZ-RCC18 had three copies. Northern blot analysis showed that GYLZ-RCC18 cDNA expressed highly in RCC, but no signal could be detected in normal kidney. Using SMART RACE technique, the full length of novel gene of GYLZ-RCC18 was obtained.
Conclusions:
The highly efficient cDNA subtractive library may have formed solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel differentially expressed genes may provide an important clue for studying the mechanism of occurrence and development of RCC.
Insights
Researchers identified novel genes differentially expressed in renal cell carcinoma (RCC) using suppression subtractive hybridization. One new gene, GYLZ-RCC18, was highly expressed in RCC tumors, offering potential targets for cancer research.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Context:
- Renal cell carcinoma (RCC) is a significant global health concern with complex molecular underpinnings.
- Identifying novel genes involved in RCC pathogenesis is crucial for developing targeted therapies.
- Current understanding of RCC-specific gene expression profiles remains incomplete.
Purpose:
- To identify and clone novel genes exhibiting differential expression in renal cell carcinoma (RCC) compared to normal kidney tissue.
- To establish a high-efficiency cDNA subtractive library for screening RCC-specific genes.
- To characterize the expression patterns of newly identified genes in RCC.
Summary:
- Suppression subtractive hybridization was employed to construct a cDNA library enriched for transcripts overexpressed in RCC.
- The library yielded 350 positive clones, with sequence analysis revealing previously unknown genes.
- The novel gene GYLZ-RCC18 was found to be highly expressed in RCC tissues but absent in normal kidney tissue, as confirmed by Northern blot analysis.
Impact:
- The developed cDNA library provides a valuable resource for the discovery of novel oncogenes and tumor suppressor genes in RCC.
- The identification of GYLZ-RCC18 offers a potential biomarker and therapeutic target for renal cell carcinoma.
- These findings contribute to a deeper understanding of the molecular mechanisms driving RCC development and progression.
