[Significance of expression and secretion of interleukin-8 in ependymal tumor]

To detect the expression and secretion of IL-8 in the ependymal tumor tissue and in primarily cultured cells, and to explore the relationship between IL-8 and ependymal tumor.

Expression of IL-8 in 50 ependymal tumor specimens was examined by immunohistochemistry. The ependymal tumor tissue obtained during operation was primarily cultured and IL-8 secreted by cultured cells were examined by clouble antibody 5 and which ELISA. The relations of expression of IL-8 to ependymal tumors of different histological degree, and invasiveness was analyzed statistically.

All the ependymal tumor tissue expressed IL-8, but the number of IL-8 positive cells in the anaplastic and malignant ependymoma was evidently larger than that in ependymoma (P < 0.01). All the cells cultured from the ependymal tumor secreted IL-8, but the liquid supernatant of anaplastic and malignant ependymoma cells contained more IL-8 than that of ependymoma cells (P < 0.01). The expression and secretion of IL-8 had no close relation to age and sex of patient.

The expression and secretion of IL-8 may be involved in the progression of ependymal tumor, so IL-8 can be used as an indicator to detect the malignant degree of ependymal tumor.