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[Effect of aldose reductase expression by transforming growth factor-beta1 on rat mesangial cell]
1Department of Pathology, Medico Collegian Fudan University, Shanghai 200032, China.
Objective:
To screen the responsive genes related to transforming growth factor-beta1 (TGF-beta1) in rat mesangial cell (MsC) transfected by human TGF-beta1 gene, using suppression subtractive hybridization PCR (SSH-PCR); then to detect the expression of aldose reductase gene on cultured rat MsC and renal cortex of rats with anti-Thy1 glomerulonephritis.
Methods:
SSH-PCR technique and differential screening for detecting the responsive genes related to TGF-beta1; Semi-quantification RT-PCR and Western blot analysis for identifying the expression of aldose reductase on MsC in vitro and in vivo.
Results:
Compared with normal MsC, it was proved that 28 responsive genes related to TGF-beta1 in MTG1. There was an expression sequence tag (EST), approximate 337 bp segment, completely identical to the data of aldose reductase gene from GenBank. Elevated levels of aldose reductase mRNA and protein were found on cultured rat MsC treated with TGF-beta1 and renal cortex of rat with anti-Thy1 glomerulonephritis.
Conclusion:
Aldose reductase, rate-limiting enzyme in the polyol pathway, was closely related to TGF-beta1 expression. It is possible that upregulation of aldose reductase expression may be involved in the development of glomerulosclerosis mediated by TGF-beta1.