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Updated: Aug 19, 2026
Genomic Transformation of the Picoeukaryote Ostreococcus tauri
Published on: July 13, 2012
[Construction of Cryptococcus neoformans cap70 transformation system]
1Department of Dermatology, Changzheng Hospital, The Second military Medical University, Shanghai 200003, China.
Objective:
To construct a transformation system of Cryptococcus neoformans capsule-deficient strain cap70.
Methods:
(1)Obtained ura5 mutants by screening the Cryptococcus neoformans cap70 using 5-fluoroorotic acid counter selection method, analyzed the obtaining strain by Southern blot and PCR; (2)Through the plasmid pCXJ18 and pCXJU which contained ura5 gene from Cryptococcus neoformans and ura3 from Kluyveromyces fragilils. as a selective marker, the ura5 strains were transformed by electrotransformation and chemical transformation.
Results:
Obtained ura5 mutants, they were detected as lack of ura function. The chemical transformation method was first be used in Cryptococcus neoformans transformation.
Conclusion:
A transformation system of Cryptococcus neoformans cap70 has been established, which provides not only the basis for gene cloning from the species for studying gene function and gene expression.
