Related Experiment Videos
Regulated gene expression in the chicken embryo by using replication-competent retroviral vectors
Noboru Sato1, Kenji Matsuda, Chie Sakuma
1Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. nsato@fmu.ac.jp
Journal of Virology
|January 19, 2002
Summary
Rous sarcoma virus (RSV)-derived vectors efficiently deliver green fluorescent protein (GFP) into chicken embryos. These vectors also enable doxycycline-inducible gene expression in ovo.
Area of Science:
- Molecular Biology
- Gene Therapy
- Developmental Biology
Background:
- Retroviral vectors are crucial tools for gene delivery in developmental studies.
- Targeted gene expression is essential for understanding cellular functions in vivo.
Purpose of the Study:
- To evaluate the efficacy of Rous sarcoma virus (RSV)-derived retroviral vectors for gene delivery in chicken embryos.
- To investigate the potential for inducible gene expression using tet regulatory elements in ovo.
Main Methods:
- Utilized RSV-derived retroviral vectors to deliver the green fluorescent protein (GFP) gene.
- Employed the cytomegalovirus (CMV) enhancer/promoter for gene expression.
- Incorporated tet regulatory elements for inducible control.
- Administered vectors to chicken embryos in ovo.
Main Results:
- RSV-derived vectors efficiently delivered GFP into restricted cell populations within the chicken embryo.
- Doxycycline administration resulted in inducible GFP expression.
Conclusions:
- RSV-derived retroviral vectors are effective for gene delivery in chicken embryos.
- Tet-inducible systems can control gene expression in ovo, offering a valuable tool for developmental research.