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Simultaneous pancreaticoduodenal-kidney transplantation in rats
G Yongping1, G Jianyun, L Jieshou
1Transplantation Center, Nanjing General Hospital, Nanjing University School of Medicine, Nanjing, People's Republic of China. guyongping@yahoo.com
Aims:
To establish a stable and simple simultaneous pancreaticoduodenal-kidney transplantation model in rats.
Methods:
Pancreaticoduodenal-kidney (left) and 1 cm of the inferior vena cava (IVC) with 0.5 cm left and right iliac communis vein were harvested from donors. We performed 'cuff' anastomoses between (1) portal vein and right iliac communis vein and (2) left kidney vein and left iliac communis vein, converging donor portal vein and left kidney vein into IVC together. Next, we performed an anastomosis of donor arterial segment and recipient abdominal aorta and a 'cuff' anastomosis between donor IVC and recipient left kidney vein.
Results:
Of 40 transplanted rats in which diabetes was induced, 33 survived over 7 days, and 31 rats have survived over 4 months. 30 rats' nonfasting plasma glucose levels were euglycemic.
Conclusions:
We performed three 'cuff' anastomoses to simplify the surgical procedure and to shorten the ischemic period of the graft. The recipient vein system has an integrated membrane to avoid thrombi in venous anastomosis sites, enhancing the transplantation success rate.