Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Continuous evaluation of Pa,O2 in fish: recording and data processing.

A Belaud, Y Trotter, C Peyraud

    The Journal of Experimental Biology
    |October 1, 1979
    PubMed
    Summary

    Researchers developed a data processing method to accurately measure blood oxygen levels in eels. This study reveals that eel breathing patterns are not solely triggered by low blood oxygen, offering new physiological insights.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    TMS reveals inhibitory extrastriate cortico-cortical feedback modulation of V1 activity in humans.

    Brain structure & function·2019
    Same author

    Effects of growth hormone on plasma ionic regulation, respiration and extracellular acid-base status in trout (Oncorhynchus mykiss) transferred to seawater.

    Fish physiology and biochemistry·2013
    Same author

    A PET study of photophobia during spontaneous migraine attacks.

    Neurology·2010
    Same author

    Distance perception within near visual space.

    Perception·2001
    Same author

    SCF(Met30)-mediated control of the transcriptional activator Met4 is required for the G(1)-S transition.

    The EMBO journal·2000
    Same author

    Gaze direction controls response gain in primary visual-cortex neurons.

    Nature·1999

    Area of Science:

    • Physiology
    • Animal Science
    • Aquatic Biology

    Background:

    • Eel (Anguilla anguilla L.) respiratory physiology involves complex ventilatory patterns.
    • Measuring blood oxygen partial pressure (PaO2) in eels presents technical challenges due to rapid physiological changes and equipment limitations.

    Purpose of the Study:

    • To develop a data processing method for accurately recording rapid PaO2 variations in eels.
    • To investigate the triggers for apnoeic (cessation of breathing) and active ventilatory phases in eels.

    Main Methods:

    • External circulatory shunt created in eels (Anguilla anguilla L.) via catheterization of dorsal aorta, ventral aorta, and subhepatic vein.
    • Continuous PO2 recording using a polarographic device, acknowledging limitations in response time.
    • Development of a data processing technique to correct for distortions in recorded PaO2 data.

    Main Results:

    • Spontaneous ventilatory interruptions in eels cause rapid PaO2 oscillations, often with periods under 1 minute.
    • The experimental setup's slow response time distorted rapid PaO2 changes, recording only slow variations (>5 min).
    • The data processing method successfully reconstituted physiological PaO2 variations, revealing that ventilatory phases are not triggered by constant low PaO2.

    Conclusions:

    • A novel data processing method enables accurate assessment of rapid PaO2 fluctuations in eels.
    • Eel breathing regulation is more complex than previously thought, not solely dependent on constant low PaO2 thresholds.
    • The developed method has potential applications in studying other physiological systems with rapid parameter changes.

    Related Experiment Videos