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Expression of MHC class I molecules together with antigenic peptides on filamentous phages
Manae Suzuki Kurokawa1, Seido Ohoka, Toshihiro Matsui
1Rheumatology, Immunology, and Genetic Program, Institute of Medical Science, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8512, Japan.
Abstract:
The availability of combinatorial T cell epitope libraries made using a phage display system would be useful for identifying the antigens recognized by T cells of unknown specificity. To this end, we have investigated here whether single chain-MHC class I molecules (scMHC-I) could be expressed together with antigenic peptides on filamentous phages. The results show that filamentous phages can express scMHC-I. Moreover, the expressed scMHC-I was able to bind antigenic peptide. These data support the use of combinatorial scMHC/T cell epitope libraries for screening potential T cell antigens.