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Related Experiment Videos

P-glycoprotein expression in mouse brain increases with maturation.

Catherine E Tsai1, Monica J Daood, Robert H Lane

  • 1Department of Pediatrics, Magee-Womens Hospital/Research Institute, University of Pittsburgh School of Medicine, 300 Halket Street, Pittsburgh, PA 15213, USA.

Biology of the Neonate
|January 23, 2002
PubMed
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P-glycoprotein (Pgp) expression in the brain is low in newborns and increases significantly with postnatal maturation. By day 21, brain Pgp levels approach adult levels, impacting blood-brain barrier function.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Developmental Biology

Background:

  • P-glycoprotein (Pgp) is a key efflux pump at the blood-brain barrier.
  • Understanding Pgp developmental expression is crucial for drug delivery and neuroprotection.

Purpose of the Study:

  • To investigate the developmental expression pattern of Pgp in mouse brain tissue.
  • To quantify Pgp mRNA and protein levels from embryonic to adult stages.

Main Methods:

  • Quantitative analysis of Pgp mRNA and protein expression.
  • Immunohistochemistry to confirm protein localization and developmental changes.
  • Expression levels were measured at embryonic day 16, and postnatal days 0, 7, 21, and in adults.

Main Results:

Related Experiment Videos

  • Pgp mRNA levels showed a significant increase with maturation, reaching 1,160% of day 0 levels in adults.
  • Pgp protein expression also increased significantly, from 52% at embryonic day 16 to 441% in adults.
  • Significant increases (p < 0.01) in both mRNA and protein were observed during postnatal development.

Conclusions:

  • Pgp expression in the mouse brain is low during late embryogenesis and the neonatal period.
  • Postnatal maturation leads to a marked increase in Pgp expression.
  • Brain Pgp protein expression approximates adult levels by postnatal day 21.