Stage determination and follow-up in sleeping sickness

In order to select a correct treatment after primary diagnosis of trypanosomiasis infection, accurate assessment of the disease stage, haemo-lymphatic or meningo-encephalitic, is essential. This is achieved by lumbar puncture and subsequent examination of the cerebrospinal fluid. These examinations have to be repeated during 2 years after treatment, and only after the cerebrospinal fluid has normalized one can decide on complete cure. The currently used cerebrospinal fluid parameters, i.e. white blood cell count, total protein determination and finding of trypanosomes, and practical problems encountered using these parameters are discussed. Alternative markers for stage determination and follow-up include trypanosome specific antibodies, anti-galactocerebroside antibodies and IgM measurement in CSF.