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Updated: Aug 4, 2026

Murine Model of CD40-activation of B cells
Published on: March 5, 2010
The expression and characterization of cyclin-dependent kinase 6 during the activation of murine G0 T-cells
T K Kwon1, J W Park, M A Buchholz
1Department of Immunology, School of Medicine, Keimyung University, Taegu, Korea. kwontk@dsmc.or.kr
Abstract:
Recent evidence strongly suggest that the D type cyclins with cdk4 and cdk6 form holoenzymes that regulate cell cycle events earlier in G1 than cyclin E/cdk2 complexes which functions near the G1/S transition. In human T lymphocytes cdk6 has been shown to be the initial retinoblastoma protein kinase detectable at mid G1. Following activation of splenic derived murine G0T-cells, cdk6, cyclin D2 and D3 specific mRNAs were detected early in G1 and reached maximal levels prior to or near G1/S. The phosphorylation of retinoblastoma protein from T-cells was detected very early in G1 and was associated mainly with cdk6/cyclin D2 complexes which accounted for a minor portion of the total cellular cdk6 contained in the cytoplasmic fraction of T-cells and mostly in the catalytically inactive form.
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