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Creatine kinase B is a target molecule of reactive oxygen species in cervical cancer
1Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
Abstract:
Recently, a procedure for detecting ROS-sensitive proteins that contain active cysteine residues was devdoped. The method is based on the fact that biotin-conjugated iodoacetamide (BIAM) and ROS competitively and selectively react with the active cysteine residues in ROS-sensitive proteins. To investigate the role of ROS in cervical cancer, BIAM labeling on cytosolic proteins in normal and cancer tissues was performed, respectively. The BIAM labeling proteins are separated by 2-dimensional electrophoresis, and then identified by MALDI-TOF mass analysis. ROS-sensitive protein is identified as creatine kinase B containing cysteine residue in active center. Activity of creatine kinase B in normal tissue is higher than that of oxidized form in cervical cancer tissues. The result suggests that ROS play an important role in metabolic regulation in cervical cancer cells. However, molecular mechanisms that ROS and creatine kinase B are integrated into a physiological signal leading to the cellular transformation remain to be elucidated.
Insights
Reactive oxygen species (ROS) are crucial in cervical cancer metabolic regulation. A new method identified creatine kinase B as a key ROS-sensitive protein, revealing its altered activity in cancer tissues.
Area of Science:
- Biochemistry
- Oncology
- Proteomics
Background:
- Reactive oxygen species (ROS) play a role in cellular processes, including cancer development.
- Identifying ROS-sensitive proteins is crucial for understanding cancer biology.
- Cervical cancer involves complex metabolic dysregulation.
Purpose of the Study:
- To develop and apply a method for detecting ROS-sensitive proteins in cervical cancer.
- To identify specific ROS-sensitive proteins involved in cervical cancer metabolism.
- To investigate the role of creatine kinase B in cervical cancer.
Main Methods:
- Utilized biotin-conjugated iodoacetamide (BIAM) labeling to detect ROS-sensitive proteins with active cysteine residues.
- Separated BIAM-labeled cytosolic proteins using 2-dimensional electrophoresis.
- Identified proteins using MALDI-TOF mass analysis.
Main Results:
- Creatine kinase B was identified as a ROS-sensitive protein containing an active cysteine residue.
- Activity of creatine kinase B was found to be higher in normal tissues compared to oxidized forms in cervical cancer tissues.
- These findings suggest ROS involvement in the metabolic regulation of cervical cancer cells.
Conclusions:
- ROS significantly influence metabolic regulation in cervical cancer.
- Creatine kinase B is a key ROS-sensitive protein implicated in cervical cancer.
- Further research is needed to elucidate the molecular mechanisms integrating ROS and creatine kinase B in cervical cancer pathogenesis.