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Peptide differential display of serum-free conditioned medium from cancer cell lines
Kae Sato1, Kazuki Sasaki, Ming-Sound Tsao
1Growth Factor Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
Cancer Letters
|January 24, 2002
Abstract:
Conditioned medium (CM) from cultured cells is a source for screening small peptides of therapeutic or diagnostic value in cancer research. Mass spectrometry has recently enabled the profiling of peptides present in biological samples. We report a single-step extraction method to increase a chance to discover small peptides with a starting volume of 750 microl of serum-free CM. In combination with protein chip mass spectrometry, our protocol will contribute to the discovery of target peptides.