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Related Experiment Videos

Review of phosphate methodologies.

E S Baginski, E Epstein, B Zak

    Annals of Clinical and Laboratory Science
    |September 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    This review details serum phosphate determination methods, recommending a simplified, sensitive, and accurate procedure using ascorbic acid and a citrate-arsenite reagent. This method effectively handles interferences like jaundice, hemolysis, and lipemia for reliable inorganic phosphate measurement.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Extensive literature exists on serum phosphate determination methodologies.
    • Analytical techniques have evolved significantly over the past century.
    • Previous methods often lacked simplicity, sensitivity, or accuracy.

    Purpose of the Study:

    • To review the historical evolution of serum phosphate determination techniques.
    • To present a simplified, sensitive, and accurate method for inorganic phosphate measurement in serum.
    • To detail reaction characteristics and potential errors of the recommended procedure.

    Main Methods:

    • Review of analytical methodologies for serum phosphate determination.
    • Development and description of a direct reaction procedure for inorganic phosphate.

    Related Experiment Videos

  • Utilizing ascorbic acid as a reducing agent and a citrate-arsenite mixture.
  • Employing a semi-aqueous medium with dimethysulfoxide and Teepol 610.
  • Investigating interference mitigation strategies, including reagent addition sequence reversal for lipemia.

    • Main Results:

    • A simplified, sensitive, and accurate procedure for serum inorganic phosphate determination is recommended.
    • The method effectively overcomes interferences from jaundice, mild hemolysis, and severe lipemia.
    • Reagent addition sequence reversal provides an idealized correction for irrelevant absorption in turbid samples.
    • Reaction characteristics and potential errors associated with the procedure are discussed.

    Conclusions:

    • The recommended method offers a robust and reliable approach for serum inorganic phosphate analysis.
    • The procedure's ability to manage common interferences enhances its clinical applicability.
    • This work contributes to the advancement of accurate and efficient diagnostic testing in clinical chemistry.