Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple hematopoietic defects and delayed globin switching in Ikaros null mice.

Rocio A Lopez1, Stuti Schoetz, Kathryn DeAngelis

  • 1Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Proceedings of the National Academy of Sciences of the United States of America
|January 24, 2002
PubMed
Summary

Ikaros is essential for forming the PYR chromatin remodeling complex in hematopoietic cells. Its absence causes severe blood cell defects and abnormal gene expression, highlighting its critical role in blood development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and optimisation of rapid real-time PCR assays for the detection of key Culicoides species.

Medical and veterinary entomology·2026
Same author

Paramedic-reported infection prevention and control practices in Canadian paramedic services before and during COVID-19.

American journal of infection control·2025
Same author

Molecular characterisation of common Culicoides biting midges (Diptera: Ceratopogonidae) in Ireland.

Parasites & vectors·2025
Same author

A risk assessment study of SARS-CoV-2 propagation in the manufacturing of cellular products.

Regenerative medicine·2022
Same author

Biomechanical Comparison of 4 Transsacral Fixation Constructs in a Type 61C, Zone II Pelvic Fracture Model.

Journal of orthopaedic trauma·2022
Same author

Pharmacists' views on the impact of the Falsified Medicines Directive on community pharmacies: A cross-sectional survey.

Exploratory research in clinical and social pharmacy·2022

Area of Science:

  • Hematopoiesis
  • Chromatin remodeling
  • Molecular biology

Background:

  • The PYR complex, a chromatin remodeling complex, contains Ikaros as its DNA binding subunit.
  • Ikaros is specifically found in adult murine and human hematopoietic cells.

Purpose of the Study:

  • To investigate the role of Ikaros in the formation and function of the PYR complex in vivo.
  • To determine the consequences of Ikaros deficiency on hematopoietic cell development and gene expression.

Main Methods:

  • Analysis of Ikaros "knockout" (null) mice (homozygous and heterozygous).
  • Assessment of PYR complex levels in Ikaros null mice.
  • Evaluation of hematopoietic cell defects, including anemia and megakaryocytic abnormalities.
  • Studies on beta-globin switching.

Related Experiment Videos

  • cDNA array analyses for gene expression profiling.

    • Main Results:

    • Homozygous Ikaros null mice lack the PYR complex, confirming Ikaros's requirement for its formation.
    • Heterozygous Ikaros null mice show reduced PYR complex levels, indicating a dosage effect.
    • Ikaros null mice exhibit multiple hematopoietic defects (anemia, megakaryocytic, lymphoid, stem cell issues).
    • Delayed beta-globin switching observed in Ikaros null mice.
    • Altered expression of hematopoietic cell-specific genes in Ikaros null embryos.

    Conclusions:

    • Ikaros is indispensable for PYR complex formation on DNA.
    • Ikaros and the PYR complex collaborate in vivo to regulate numerous adult hematopoietic cell-specific genes.
    • Disruption of Ikaros function leads to pleiotropic hematopoietic defects due to dysregulated gene expression.