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Related Experiment Videos

Actin cable dynamics in budding yeast.

Hyeong-Cheol Yang1, Liza A Pon

  • 1Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.

Proceedings of the National Academy of Sciences of the United States of America
|January 24, 2002
PubMed
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Budding yeast actin cables are essential for cell polarity. New bud-associated actin cables form within the bud and elongate, rather than realigning existing structures, to establish cell polarity.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Budding Yeast Models

Background:

  • Actin cables, aligned actin filaments, are vital for establishing cell polarity in budding yeast.
  • Understanding actin cable dynamics is key to deciphering cell polarity mechanisms.

Purpose of the Study:

  • To visualize and analyze the dynamic behavior of actin cables in living budding yeast.
  • To elucidate the mechanisms of actin cable formation and elongation during the cell cycle.

Main Methods:

  • Utilized green fluorescent protein (GFP) tagging of actin binding protein 140 (Abp140p) for live-cell imaging.
  • Employed time-lapse microscopy and fluorescence loss in photobleaching experiments.
  • Investigated the effects of Latrunculin A (Lat-A) on actin cable dynamics.

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Main Results:

  • Identified two actin cable populations: bud-associated and randomly oriented.
  • Demonstrated that bud-associated actin cables elongate via new assembly at the bud end and movement of the opposite end.
  • Observed movement of randomly oriented cables and noted Lat-A's effect on their numbers but not velocity.

Conclusions:

  • Bud-associated actin cable establishment involves de novo assembly and elongation, not realignment.
  • Actin cable dynamics are crucial for cell polarity establishment in budding yeast.
  • Differential responses of cable populations to Latrunculin A suggest distinct regulatory mechanisms.