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Detection of mycobacterium tuberculosis by nested polymerase chain reaction in a case of subconjunctival tuberculosis
Jyotirmay Biswas1, S Krishna Kumar, Pankaj Rupauliha
1Medical & Vision Research Foundations, Sankara, Nethralaya Chennai, India. mrf@sankaranethralaya.org
Purpose:
To highlight the importance of nested polymerase chain reaction (PCR) in the detection of Mycobacterium tuberculosis in a case of subconjunctival tuberculosis.
Methods:
We report a case of a 60-year-old man with subconjunctival nodule in the right eye for duration of 6 weeks. Biopsy of the nodule showed a granuloma with extensive caseation necrosis. Ziehl Neelsen staining for acid-fast bacilli (AFB) was negative. However, because of a strong suspicion of Mycobacterium infection, PCR for M. tuberculosis genome was done, using the nested PCR technique.
Results:
Polymerase chain reaction for M. tuberculosis showed amplification of Mycobacterium tuberculosis genome with the nested PCR technique.
Conclusion:
Our case indicates that PCR can be a valuable tool in the diagnosis of conjunctival tuberculosis from paraffin sections.
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