Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility
H B Dincturk1, A J Dunn, D H Jones
1Molecular Biology Research Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK. dincturk@itu.edu.tr
Journal of Biosciences
|January 25, 2002
Abstract:
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
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