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A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features
1Women's General Hospital, IVF-Unit, Linz, Upper Austria, Austria. thomas.ebner@li.lkh.ooe.gv.at
Purpose:
To determine the influence of technical pitfalls and oocyte morphology on survival rate and cleavage behavior after ICSI.
Methods:
A total of 2210 injection procedures was examined for morphological and technical deviations. Survival rate and cleavage behavior were evaluated.
Results:
In 77.8% of all cases ICSI was unsuspicous. Out of 491 deviations from optimal injection deep penetration of the oocyte and abundant presence of cumulus cells showed significant correlation with degeneration rate (p < 0.001). Morphological anomalies associated with the periphery of the oocyte were rather related to degeneration than cytoplasmic anomalies (p < 0.001). Early embryonic development was not impaired by technical or morphological parameters.
Conclusions:
To conclude, these prospective data may be of prognostic value in regard of the number of embryos available for transfer and may help to improve treatment outcome.