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Related Experiment Videos

Function of the tetraspanin CD151-alpha6beta1 integrin complex during cellular morphogenesis.

Xin A Zhang1, Alexander R Kazarov, Xiuwei Yang

  • 1Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Molecular Biology of the Cell
|January 26, 2002
PubMed
Summary

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The CD151-alpha6beta1 integrin complex is crucial for cell network formation and morphogenesis. The CD151 tail specifically regulates alpha6beta1 integrin function, impacting cellular behavior and potentially angiogenesis.

Area of Science:

  • Cell Biology
  • Integrin Signaling
  • Extracellular Matrix Interactions

Background:

  • NIH3T3 cells form cord-like networks on Matrigel, a process influenced by alpha6beta1 integrin.
  • CD151 protein is implicated in cell adhesion and migration, but its precise role in integrin-mediated morphogenesis is unclear.

Purpose of the Study:

  • To investigate the functional role of the CD151-alpha6beta1 integrin complex in NIH3T3 cell network formation.
  • To determine the importance of the CD151 C-terminal tail in regulating alpha6beta1 integrin activity.

Main Methods:

  • NIH3T3 cell culture on Matrigel.
  • Transfection of NIH3T3 cells with wild-type and mutant CD151.
  • Inhibition studies using anti-alpha6beta1 and anti-CD151 antibodies.
  • Assessment of cell network formation and cell spreading assays.

Related Experiment Videos

  • Co-immunoprecipitation to confirm protein association.

    • Main Results:

    • NIH3T3 cell network formation on Matrigel was inhibited by anti-alpha6beta1 and anti-CD151 antibodies.
    • CD151 and alpha6beta1 integrin were found to be physically associated.
    • Exogenous CD151 with a deleted C-terminal tail suppressed alpha6beta1-dependent cell network formation and spreading.
    • Mutant CD151 retained alpha6beta1 association but did not affect ligand-independent cell adhesion.

    Conclusions:

    • The CD151-alpha6beta1 integrin complex functions as a unit influencing cellular morphogenesis.
    • The CD151 C-terminal tail is critical for the "outside-in" signaling of alpha6beta1 integrin after ligand engagement.
    • CD151-alpha6beta1 complexes may play a role in endothelial cell network formation during angiogenesis.