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Related Experiment Videos

FCCP depolarizes plasma membrane potential by activating proton and Na+ currents in bovine aortic endothelial cells.

Kyu-Sang Park1, Inho Jo, Kim Pak

  • 1Division of Metabolic Disease, Dept. of Biomedical Sciences, National Institute of Health, 5 Nokbun-Dong, Eunpyung-Ku, Seoul 122-701, Korea.

Pflugers Archiv : European Journal of Physiology
|January 26, 2002
PubMed
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Carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) affects plasma membrane potential and ionic currents in bovine aortic endothelial cells (BAECs). FCCP-induced currents are mediated by both proton (H+) and sodium (Na+) transport, influenced by the proton gradient.

Area of Science:

  • Cellular physiology
  • Ion transport mechanisms
  • Mitochondrial function

Background:

  • Mitochondrial uncouplers like carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) can influence cellular electrophysiology.
  • The precise mechanisms of FCCP's effects on plasma membrane potential and ionic currents in endothelial cells are not fully elucidated.
  • Endothelial cell function is critically dependent on ion gradients and membrane potential.

Purpose of the Study:

  • To investigate the effects of FCCP on plasma membrane potential and ionic currents in bovine aortic endothelial cells (BAECs).
  • To determine the ionic basis and the role of proton (H+) and sodium (Na+) gradients in FCCP-induced cellular responses.
  • To explore the relationship between FCCP, protonophores, and endothelial cell ion transport.

Main Methods:

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  • Utilized the patch-clamp technique in current-clamp and voltage-clamp modes to record membrane potential and ionic currents in BAECs.
  • Manipulated extracellular and intracellular pH, extracellular Na+ concentration, and intracellular Ca2+ levels.
  • Analyzed FCCP-induced currents and their dependence on ion gradients and pH.

Main Results:

  • FCCP dose-dependently activated ionic currents and depolarized the plasma membrane potential in BAECs.
  • FCCP-induced currents were independent of intracellular Ca2+ changes but strongly influenced by extracellular and intracellular pH.
  • FCCP-induced currents were reduced in Na+-free conditions, indicating significant Na+ involvement, alongside H+ transport.

Conclusions:

  • FCCP-induced ionic currents and plasma membrane depolarization in BAECs are primarily mediated by the plasmalemmal proton gradient.
  • Both proton (H+) and sodium (Na+) currents contribute to FCCP-induced electrophysiological changes.
  • The interplay between H+ and Na+ transport in response to FCCP requires further investigation.