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[Effect of thrombopoietin II on exsanguine thrombocytopenia mouse death rate]
1Department of Hematology, First Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China.
Objective:
To study the effect of thrombopoietin II (TPO II) on the exsanguine thrombocytopenia mouse death rate.
Methods:
After the normal peripheral platelet counts were done on the samples obtained from the tail vein of purebred Babl/c mice before experiment, the purified ligand I of TPO II, artificial compound ligand II of TPO II and rhTPO were injected intraperitoneally once a day for 7 days. On d 7 and d 14, platelet counts were performed on 0.5 ml samples obtained from the supra-orbital vein, with the condition of the mouse death monitored daily.
Results:
On d 7, ligand I of TPO II group platelet counts were higher than that of the negative control group (P < 0.05), while not being significantly different from that of rhTPO group (P > 0.05). On d 14, the platelet counts of two TPO II groups increased significantly as compared with the negative control group (P < 0.01), showing no significant difference from that of rhTPO group (P > 0.05). Moreover, the platelet counts of mice in two TPO II groups and the positive group had shown an increasing tendency in the days following experiment. In addition, mouse death occurred in all groups of mice following their phlebotomy from the supraorbital vein on d 7. But the death rate of negative control group was evidently higher than that of any other groups (P < 0.05).
Conclusion:
TPO II's biological activity obviously increases platelet production, thereby reducing the exsanguine thrombopenia mouse death rate.
