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Purification and characterization of a 29 kDa poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl
V Cheriyath1, A Balasubrahmanyam, H C Kapoor
1Division of Biochemistry, Indian Agricultural Research Institute, New Delhi.
Indian Journal of Biochemistry & Biophysics
|January 29, 2002
Abstract:
A poly(A)-binding protein (PABP) with mol wt 29,000 has been purified from chickpea (Cicer arietinum) epicotyl by ammonium sulfate fractionation and Cibacron blue F3-GA chromatography, making a complex with poly(A) and elution of PABP-poly(A) complex at 45 degrees C from oligo d(T)-cellulose. The elution pattern and binding properties show that the purified protein is different from the PABP (mol. wt 72,000) reported earlier from our laboratory.