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Related Experiment Videos

A parallel processing solid phase extraction protocol for the determination of whole blood folate.

Yumei Lin1, Stephen R Dueker, A Daniel Jones

  • 1Department of Nutrition, University of California, Davis, California 95616, USA.

Analytical Biochemistry
|January 29, 2002
PubMed
Summary

This study presents an enhanced whole blood folate analysis method for higher throughput. The improved technique offers accurate folate measurements, crucial for clinical and epidemiological research.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Accurate whole blood folate analysis is essential for clinical and epidemiological studies.
  • Previous methods faced limitations in throughput and potential interferences.

Purpose of the Study:

  • To describe an improved, high-throughput method for whole blood folate analysis.
  • To enhance accuracy and reduce interferences in folate measurement.

Main Methods:

  • Implemented a buffered solvent exchange to remove interfering amino acids like phenylalanine.
  • Replaced HPLC with NH2 solid phase extraction for parallel sample processing.
  • Utilized heptafluorobutyl derivatives instead of trifluoroacetyl derivatives for better GC resolution.

Main Results:

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  • Achieved relative standard deviations typically of 5% or less.
  • Enabled a single operator to process batches of 40 samples.
  • Demonstrated strong correlation (R = 0.98) with the Lactobacillus casei assay for whole blood folate.

    • Conclusions:

    • The modified method significantly increases throughput for whole blood folate analysis.
    • The internal standardization minimizes interlaboratory variation, ensuring reliable results.
    • This method is suitable for large-scale epidemiological and clinical studies requiring precise folate quantification.