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Related Experiment Videos

A permissive apoptotic environment: function of a decrease in intracellular superoxide anion and cytosolic

Shazib Pervaiz1, Marie-Véronique Clément

  • 1Department of Physiology, National University of Singapore, Singapore. phssp@nus.edu.sg

Biochemical and Biophysical Research Communications
|January 29, 2002
PubMed
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Decreased intracellular superoxide levels and cytosolic acidification create a permissive environment for cell death. This balance of reactive oxygen species is critical for regulating apoptosis and cellular responses to death stimuli.

Area of Science:

  • Cellular biology
  • Biochemistry
  • Oxidative stress

Background:

  • Reactive oxygen species (ROS) play diverse roles in cellular processes, including proliferation and cell death.
  • Physiological concentrations of ROS, like superoxide anion, can inhibit apoptosis signaling.
  • Maintaining a balance between intracellular superoxide and hydrogen peroxide is crucial for cellular function.

Purpose of the Study:

  • To investigate the role of intracellular superoxide concentration and cytosolic pH in regulating cellular sensitivity to apoptosis.
  • To elucidate the mechanism by which shifts in reactive oxygen species balance impact cell death signaling.

Main Methods:

  • Cellular assays to measure reactive oxygen species levels.
  • Apoptosis induction and sensitivity assays.

Related Experiment Videos

  • Monitoring of cytosolic pH changes.

    • Main Results:

    • Decreased intracellular superoxide concentration significantly increases cellular sensitivity to apoptotic triggers.
    • A shift in the balance towards hydrogen peroxide leads to activation of apoptotic machinery.
    • This shift induces intracellular milieu reduction and cytosolic acidification, facilitating cell death execution.

    Conclusions:

    • A permissive apoptotic milieu is characterized by decreased intracellular superoxide concentration and cytosolic acidification.
    • The balance between superoxide and hydrogen peroxide is a critical determinant of cellular response to death stimuli.
    • Understanding this balance offers insights into regulating cell death pathways.