Related Experiment Video
Updated: Aug 11, 2026
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
[Frequent loss of fragile histidine triad gene transcripts in lung cancer]
1Department of Medical Oncology, National Laboratory of Molecular Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Objective:
To investigate the significance of the fragile histidine triad (FHIT) gene in evaluation of early diagnosis and metastasis in lung cancer.
Methods:
47 lung cancer samples and 16 matched metastatic hilar lymph nodes of lung cancer patients were examined for abnormalities of the gene by using RT-PCR and RT-PCR-SSCP.
Results:
32 of 47 (68%) lung cancer samples and 15 of 16 metastatic hilar lymph nodes showed loss of FHIT gene transcripts (exon5-9 or exon1-4). RT-PCR-SSCP reveal no variants.
Conclusions:
These data indicate that loss of FHIT gene transcript is frequent in lung cancer and may be an early event in lung tumorigenesis and FRA3B may be involved in the development and progression of the lung cancer.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
01:12Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...