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[The alteration of microsatellite DNA in lung cancer]
Objective:
To study the alteration of microsatellite DNA in primary lung cancer and its value of diagnosis for lung cancer.
Methods:
Five microsatellite markers on chromosome 3P13-26 have been examined in 50 primary lung cancer tissues and their normal lung tissues.
Results:
In fifty lung cancer, the incidence of microsatellite instability (MSI) was 52% (26/50) and the loss of heterozygosity (LOH) 38% (19/50). The positive rate of MSI in poorly differentiated lung cancer (13/17, 77%) was significantly higher than that in well, moderately differentiated (10/28, 36%). The sensitivity in the diagnosis of lung cancer was 72% by combining MSI and LOH.
Conclusions:
MSI and LOH may play a certain role in the carcinogenesis and progression of lung cancer and they may be valuable molecular markers in diagnosis of lung cancer.