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RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA)
B Verbeure1, E Lescrinier, J Wang
1Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Nucleic Acids Research
|January 29, 2002
Abstract:
Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.