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Related Experiment Videos

Gene expression in the developing mouse retina by EST sequencing and microarray analysis.

X Mu1, S Zhao, R Pershad

  • 1Department of Biochemistry and Molecular Biology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.

Nucleic Acids Research
|January 29, 2002
PubMed
Summary
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Researchers analyzed expressed sequence tags (ESTs) from mouse retinal development to understand gene networks. This identified over 5,000 genes, revealing a higher proportion involved in eye development and a link between Brn-3b and GAP-43.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genomics

Background:

  • Retinal development involves neuroblast differentiation into retinal cells between embryonic day E11.5 and post-natal day P8.
  • Understanding the global gene networks and complexity of gene sets regulating retinal development is limited.

Purpose of the Study:

  • To perform sequence analysis of a mouse retinal cDNA library at embryonic day E14.5.
  • To identify expressed genes and their functions during retinal development.
  • To investigate potential regulatory linkages between genes, such as Brn-3b and GAP-43.

Main Methods:

  • Sequence analysis of an E14.5 retinal cDNA library, archiving 15,268 expressed sequence tags (ESTs).
  • Annotation of 9,035 ESTs, representing 5,288 genes, and functional categorization.

Related Experiment Videos

  • Microarray screening using wild-type and brn-3b (-/-) retinal cDNA probes.

    • Main Results:

    • Archived 15,268 ESTs, annotated 9,035 representing 5,288 genes, with an estimated 27,000 total expressed genes.
    • Found a significantly higher representation of eye development genes in the retinal clone set compared to a standard mouse cDNA set.
    • Revealed a potential regulatory link between the transcription factor Brn-3b and GAP-43 expression, a protein involved in axon growth.

    Conclusions:

    • The generated retinal EST database is a valuable resource for gene expression profiling and gene discovery.
    • The study provides insights into the complexity of the gene set required for retina formation.
    • Identified a potential regulatory role for Brn-3b in retinal axon development via GAP-43.