Jove
Visualize
Contact Us

Related Experiment Videos

A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'.

A Kesminiene1, Z Maneliene, J Vitkute

  • 1MBI Fermentas, and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.

Nucleic Acids Research
|January 29, 2002
PubMed
Summary

Researchers discovered a new enzyme, FspAI, from Flexibacter species. This type II restriction endonuclease recognizes and cleaves an 8-base DNA sequence, producing blunt DNA ends.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The State Scientific Automated Medical Registry, Kazakhstan: an important resource for low-dose radiation health research.

Radiation and environmental biophysics·2018
Same author

Cancer risk from paediatric computed tomography scanning: implications for radiation protection in medicine.

Annals of the ICRP·2018
Same author

Dose Estimation for a Study of Nuclear Workers in France, the United Kingdom and the United States of America: Methods for the International Nuclear Workers Study (INWORKS).

Radiation research·2015
Same author

A framework for estimating radiation-related cancer risks in Japan from the 2011 Fukushima nuclear accident.

Radiation research·2014
Same author

AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3'.

Nucleic acids research·2002
Same author

Studies of cancer risk among Chernobyl liquidators: materials and methods.

Journal of radiological protection : official journal of the Society for Radiological Protection·2002
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Enzymology
  • Genomics

Background:

  • Restriction endonucleases are crucial tools in molecular biology for DNA manipulation.
  • Type II restriction enzymes recognize specific DNA sequences and cleave them, often generating blunt or sticky ends.
  • The characterization of novel restriction enzymes expands the toolkit for genetic engineering and analysis.

Purpose of the Study:

  • To identify and characterize a novel type II restriction endonuclease from a Flexibacter species.
  • To determine the DNA recognition sequence and cleavage site of the new enzyme, designated FspAI.
  • To assess the potential utility of FspAI in molecular biology applications.

Main Methods:

  • Partial purification of the FspAI enzyme from Flexibacter species Tv-m21K.

Related Experiment Videos

  • DNA substrate assays to determine the recognition sequence and cleavage pattern.
  • Analysis of DNA cleavage products to confirm blunt-end generation.

    • Main Results:

    • A new type II restriction endonuclease, FspAI, was partially purified.
    • FspAI recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'.
    • The enzyme cleaves the recognition site at the center, producing blunt-ended DNA fragments.

    Conclusions:

    • FspAI represents a novel restriction enzyme with a unique octanucleotide recognition site.
    • The blunt-ended fragments generated by FspAI are valuable for DNA ligation and cloning applications.
    • Further characterization of FspAI could lead to its application in various molecular biology techniques.