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A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'
A Kesminiene1, Z Maneliene, J Vitkute
1MBI Fermentas, and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
Nucleic Acids Research
|January 29, 2002
Abstract:
A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5'-RTGC/GCAY-3' and cleaves it in the center generating blunt-ended DNA fragments.