Related Experiment Videos
Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta
Madoka Watanabe1, Shinya Hanashima, Yoshiyuki Mizushina
1Department of Applied Biological Science, Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
Bioorganic & Medicinal Chemistry Letters
|January 30, 2002
Abstract:
Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.