Jove
Visualize
Contact Us

Related Experiment Videos

Molecular modelling in structural biology.

Mark J Forster1

  • 1Informatics Laboratory, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, UK. mfoster@nibsc.ac.uk

Micron (Oxford, England : 1993)
|January 30, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The localisation of the heparin binding sites of human and murine interleukin-12 within the carboxyterminal domain of the P40 subunit.

Cytokine·2018
Same author

The potential utility of predicted one bond carbon-proton coupling constants in the structure elucidation of small organic molecules by NMR spectroscopy.

PloS one·2014
Same author

Toward interoperable bioscience data.

Nature genetics·2012
Same author

Structural characterisation, stability and antibody recognition of chimeric NHBA-GNA1030: an investigational vaccine component against Neisseria meningitidis.

Vaccine·2012
Same author

Empowering industrial research with shared biomedical vocabularies.

Drug discovery today·2011
Same author

Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments.

The Journal of biological chemistry·2005
Same journal

Immunofluorescence study and morphometric analysis of collagen-IV in the exchange tissue of the quail (Coturnix coturnix) lung by confocal laser scanning microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

Micromorphological study of leaf surfaces structures in selected Crataegus L. (Rosaceae) species using light, scanning electron, and confocal laser microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

3D reconstruction of the nymphal feeding apparatus of Philaenus spumarius.

Micron (Oxford, England : 1993)·2026
Same journal

The influence of physicians and surgeons on Leeuwenhoek's observations of crystal formation.

Micron (Oxford, England : 1993)·2026
Same journal

Distribution of telocytes in the choroid (eye) of a teleost: An ultrastructural observation.

Micron (Oxford, England : 1993)·2026
Same journal

SEM-EDAX: A tool for microanalytical elemental mapping in butterfly wing scales.

Micron (Oxford, England : 1993)·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Molecular modelling is crucial for understanding biological macromolecule structures. This review highlights its integral role in experimental structure determination and predicting novel protein folds using homology and threading methods.

Area of Science:

  • Structural Biology
  • Computational Biology
  • Biophysics

Background:

  • Molecular modelling is a key technique for analyzing the 3D structures of biological macromolecules.
  • Its application in structural biology is diverse, often playing an integral role in experimental structure determination.
  • Genome sequencing yields vast data, but many proteins lack homology to known structures, necessitating advanced modelling.

Purpose of the Study:

  • To review the multifaceted applications of molecular modelling in structural biology.
  • To emphasize the utility of modelling in interpreting experimental data and generating testable hypotheses.
  • To discuss the role of modelling in predicting protein structures from sequence, especially for novel folds.

Main Methods:

  • Homology modelling to construct 3D models based on sequence similarity.

Related Experiment Videos

  • Threading methods to identify protein folds even without clear sequence homology.
  • Methods for modelling protein-ligand and protein-protein complexes.

    • Main Results:

    • Molecular modelling aids in interpreting and rationalizing experimental data from techniques like NMR and X-ray crystallography.
    • Homology modelling and threading methods are effective for predicting structures of proteins with limited or no sequence homology.
    • Systematic structure determination efforts will reduce the number of unassigned protein folds, increasing the reliance on modelling.

    Conclusions:

    • Molecular modelling is an indispensable tool in modern structural biology, complementing experimental methods.
    • Advanced modelling techniques like homology and threading are essential for characterizing proteins from genome sequencing projects.
    • The utility of molecular modelling is expected to grow significantly with ongoing advancements and collaborative efforts.