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Individual molecules of thermostable alkaline phosphatase support different catalytic rates at room temperature
Ashley C Dyck1, Douglas B Craig
1Department of Chemistry, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada.
Luminescence : the Journal of Biological and Chemical Luminescence
|January 30, 2002
Abstract:
Thermus thermophilus cells were grown at 70 degrees C, lysed and the lysate subjected to single molecule alkaline phosphatase assays, using a capillary electrophoresis laser-induced fluorescence detection-based method. The enzyme was found to be heterogeneous with respect to catalytic rate when assayed at room temperature. Turnover numbers ranged 12-fold, with an average of 400 +/- 200 reactions/min for the 80 molecules assayed.