Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evolutionary engineering of industrially important microbial phenotypes.

U Sauer1

  • 1Institute of Biotechnology, ETH Zürich, 8093 Zürich, Switzerland. sauer@biotech.biol.ethz.ch

Advances in Biochemical Engineering/Biotechnology
|January 31, 2002
PubMed
Summary

Evolutionary engineering complements metabolic engineering by using variation and selection for strain development. This approach aids in understanding and transferring desired traits, advancing cellular engineering.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Issues relating to the release of proprietary information and data for use in the validation of alternative methods.

Alternatives to laboratory animals : ATLA·2015
Same author

Benefits of a European project on diagnostics of highly pathogenic agents and assessment of potential "dual use" issues.

Frontiers in public health·2014
Same author

Bladder cancer biomarker array to detect aberrant levels of proteins in urine.

The Analyst·2014
Same author

Cementless acetabular reconstruction and structural bone-grafting in dysplastic hips. Surgical technique.

The Journal of bone and joint surgery. American volume·2007
Same author

Cementless acetabular reconstruction and structural bone-grafting in dysplastic hips.

The Journal of bone and joint surgery. American volume·2006
Same author

Optimizing processing parameters for signal enhancement of oligonucleotide and protein arrays on ARChip Epoxy.

Bioelectrochemistry (Amsterdam, Netherlands)·2005

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Microbial Engineering

Background:

  • Cellular system complexity hinders metabolic engineering applications.
  • Evolutionary engineering offers a complementary strategy using variation and selection.
  • This method is advantageous for strain development and process optimization.

Purpose of the Study:

  • To present evolutionary engineering of whole cells as an emerging methodology.
  • To highlight its role in overcoming limitations of traditional metabolic engineering.
  • To discuss its future relevance in strain development and understanding evolved phenotypes.

Main Methods:

  • Variation and selection principles.
  • Empirical strain development via random mutation and direct selection.

Related Experiment Videos

  • Recombination and continuous evolution of large populations.
  • Global response analysis at genetic and metabolic levels.

    • Main Results:

    • Evolutionary engineering provides compelling scientific and applied advantages.
    • It enables the elucidation of molecular bases for desired phenotypes.
    • Integration with analytical techniques, bioinformatics, and modeling enhances its utility.

    Conclusions:

    • Evolutionary engineering is a powerful tool for metabolic engineering.
    • It facilitates the development of improved microbial strains.
    • It aids in understanding the genetic and metabolic underpinnings of evolved traits.