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Cyclophosphamide and related anticancer drugs
1Institute of Clinical Pharmacology, University of Leipzig, Germany. baumf@medizin.uni-leipzig.de
Journal of Chromatography. B, Biomedical Sciences and Applications
|January 31, 2002
Summary
This article reviews bioanalytical methods for oxazaphosphorines like cyclophosphamide and ifosfamide. It details the broad spectrum of assays needed due to their complex metabolism and numerous metabolites.
Area of Science:
- Pharmacology and Toxicology
- Analytical Chemistry
Background:
- Oxazaphosphorines, including cyclophosphamide, ifosfamide, and trofosfamide, are crucial chemotherapeutic agents.
- Their complex metabolism generates a wide array of active and inactive metabolites.
- Accurate bioanalysis is essential for understanding their pharmacokinetics and efficacy.
Purpose of the Study:
- To provide a comprehensive overview of bioanalytical methods for oxazaphosphorines and their metabolites.
- To highlight the diversity of analytical techniques required for this drug class.
- To describe particularly important assays in detail.
Main Methods:
- Review of various bioanalytical techniques.
- Tabulation of methods for oxazaphosphorine and metabolite quantification.
- Detailed description of key analytical assays.
Main Results:
- The complex metabolism necessitates a broad spectrum of bioanalytical methods.
- Various chromatographic and spectroscopic techniques are employed.
- Specific assays are crucial for accurate metabolite profiling.
Conclusions:
- Effective bioanalysis of oxazaphosphorines requires a diverse methodological approach.
- Understanding metabolite profiles is key to optimizing cancer therapy.
- This review serves as a guide to current bioanalytical strategies.