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Separation of finasteride and analogues
1Pharmakl spol. sro, Laboratory and Clinical Facility, U Vojenské Nemocnice, Prague, Czech Republic. pharmakl@mbox.vol.cz
Journal of Chromatography. B, Biomedical Sciences and Applications
|January 31, 2002
Summary
This review covers chromatographic methods for analyzing finasteride and related drugs in biological samples, crucial for treating benign prostatic hyperplasia. High-performance liquid chromatography with mass spectrometry is best for low concentrations, while spectrophotometry works for higher levels.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Biochemistry
Background:
- Finasteride and its analogues are key treatments for benign prostatic hyperplasia.
- Accurate quantification in biological fluids is essential for therapeutic drug monitoring and pharmacokinetic studies.
Purpose of the Study:
- To review and compare chromatographic techniques for finasteride and analogue determination.
- To discuss the relationship between pharmacokinetics, dosage, and required quantification limits.
- To guide the selection of appropriate detection methods based on concentration levels.
Main Methods:
- Focus on high-performance liquid chromatography (HPLC) with spectrophotometric and mass spectrometric detection.
- Inclusion of sample clean-up procedures.
- Discussion of pharmacokinetic data and assay requirements.
Main Results:
- Tandem mass spectrometry (MS/MS) is recommended for sensitive detection (<1 ng/ml).
- Spectrophotometric detection is a viable, cost-effective option for higher concentrations (>1 ng/ml).
- Clean-up procedures are critical for accurate quantification.
Conclusions:
- Chromatographic methods, particularly HPLC, are vital for finasteride analysis.
- Method selection depends on the required limit of quantification and cost-effectiveness.
- MS/MS offers superior sensitivity for low-concentration pharmacokinetic studies.