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Separation methods for antiviral phosphorus-containing drugs.
1Laboratoire de Pharmacocinétique et de Pharmacie Clinique, Faculté de Pharmacie, Université Victor Segalen Bordeaux 2, France. boubakar.ba@phcocin.u-bordeaux2.fr
Journal of Chromatography. B, Biomedical Sciences and Applications
|January 31, 2002
Summary
Accurate monitoring of antiviral drugs foscarnet and cidofovir is crucial due to renal toxicity. Novel HPLC methods with electrochemical or fluorescent detection enable precise quantification in biological samples for CMV retinitis treatment.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Biochemistry
Background:
- Foscarnet and cidofovir are antiviral drugs used for CMV retinitis.
- These drugs exhibit adverse effects, including renal toxicity.
- Lifelong therapy necessitates accurate drug monitoring methods.
Purpose of the Study:
- To develop and validate analytical methods for foscarnet and cidofovir.
- To enable therapeutic drug monitoring in biological samples.
- To address limitations of UV detection for these hydrophilic compounds.
Main Methods:
- Ion pair reversed-phase High-Performance Liquid Chromatography (HPLC) was employed.
- Electrochemical detection (coulometry, amperometry) was used for foscarnet.
- Fluorescent derivatization of cidofovir was performed for enhanced detection.
Main Results:
- Electrochemical detection achieved a quantification limit of 15 microM for foscarnet.
- Fluorescent derivatization of cidofovir yielded a detection limit of 5 ng/ml.
- These methods allow for drug time-course investigations in biological fluids.
Conclusions:
- Developed HPLC methods with electrochemical and fluorescent detection are suitable for monitoring foscarnet and cidofovir.
- These techniques overcome UV detection limitations for hydrophilic compounds.
- Accurate drug monitoring is essential for managing CMV retinitis and mitigating toxicity.