Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography
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M R Viant1, E S Rosenblum, R S Tjeerdema
1Department of Environmental Toxicology, University of California, Davis 95616, USA. mrviant@ucdavis.edu
Abstract:
A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH2PO4-acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (Haliotis rufescens) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2+/-0.9% extraction efficiency.
