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Related Experiment Videos

Simple inoculum standardizing system for antimicrobial disk susceptibility tests.

A L Barry, D Amsterdam, M B Coyle

    Journal of Clinical Microbiology
    |December 1, 1979
    PubMed
    Summary
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    The Inocupac system offers a convenient method for standardizing antimicrobial disk susceptibility tests, showing comparable precision to the Bauer-Kirby method for common bacteria. This system is a valid alternative for routine laboratory use.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Infectious Diseases

    Background:

    • Accurate inoculum standardization is crucial for reliable antimicrobial susceptibility testing.
    • The Bauer-Kirby method is the standard but can be time-consuming.
    • A need exists for efficient and convenient methods for inoculum standardization.

    Purpose of the Study:

    • To evaluate the Inocupac system as a device for expediting inoculum standardization in antimicrobial disk susceptibility testing.
    • To compare the performance of the Inocupac system with the standard Bauer-Kirby method.
    • To assess the inter- and intralaboratory precision and interpretive agreement of the Inocupac system.

    Main Methods:

    • A collaborative study involving five investigators.

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  • Testing of 100 selected bacterial isolates in triplicate using both the Inocupac system and the Bauer-Kirby method.
  • Parallel performance evaluation of disk susceptibility tests.

    • Main Results:

    • Inter- and intralaboratory precision of the Inocupac system was comparable to the Bauer-Kirby method.
    • The Inocupac system showed limitations with some streptococci but performed well with gram-negative bacilli, staphylococci, and most enterococci.
    • Zone sizes were similar (+/-2 mm) between the two methods for tested organisms.
    • Interpretive agreement ranged from 90% to 99% across different antimicrobial agents.

    Conclusions:

    • The Inocupac system is a valid alternative method for inoculating disk susceptibility tests.
    • The device expedites inoculum standardization, offering a convenient option for clinical laboratories.
    • Further evaluation may be needed for specific challenging organisms like certain streptococci.