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Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Jun 25, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

DNA-catalyzed polymerization.

Xiaoyu Li1, Zheng-Yun J Zhan, Rachel Knipe

  • 1Department of Chemistry, Emory University, Emerson Hall, Atlanta, Georgia 30322, USA.

Journal of the American Chemical Society
|January 31, 2002
PubMed
Summary

Native DNA oligomers catalyze stereoselective polymerization of modified nucleosides. This method translates DNA sequence information into synthetic polymers with high fidelity, selecting against mismatches.

Area of Science:

  • Biochemistry
  • Synthetic Biology
  • Polymer Chemistry

Background:

  • DNA oligomers are fundamental to genetic information storage.
  • Catalysis plays a crucial role in polymer synthesis.
  • Stereoselective synthesis is key for creating functional polymers.

Purpose of the Study:

  • To investigate the catalytic potential of native DNA oligomers.
  • To develop a method for stereoselective polymerization of modified nucleosides.
  • To establish a system for translating biopolymer information into synthetic polymers.

Main Methods:

  • Utilizing native DNA oligomers as stereoselective catalysts.
  • Employing reductive amination for the polymerization of 5'-amino-3'-acetaldehyde-modified thymidine/adenosine nucleosides.

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Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

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Last Updated: Jun 25, 2026

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Published on: August 26, 2009

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

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11:44

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

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  • Analyzing reaction kinetics and mismatch selectivity.

    • Main Results:

    • DNA oligomers demonstrated stereoselective catalysis for nucleoside polymerization.
    • The polymerization followed step-growth kinetics, reading information in the antiparallel direction.
    • High selectivity (>100:1) against single mismatches was observed.

    Conclusions:

    • A novel method for stereoselective polymer synthesis using DNA catalysts has been established.
    • This approach enables the translation of encoded sequence and chain-length information into synthetic polymers.
    • The high fidelity of the process offers potential for advanced biomaterial development.