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Analysis of lipocyte viability after liposuction
Mark T Boschert1, Benjamin W Beckert, Charles L Puckett
1Division of Plastic Surgery, University of Missouri, Columbia, 65212, USA.
Plastic and Reconstructive Surgery
|January 31, 2002
Summary
Centrifuging liposuction aspirate for two minutes at 50g stratifies fat cells. The deepest layer contains significantly more viable adipocytes, potentially improving soft-tissue augmentation graft survival.
Area of Science:
- Regenerative Medicine
- Plastic Surgery
- Adipose Tissue Engineering
Background:
- Autologous fat grafting is a common technique for soft-tissue augmentation.
- Optimizing the viability of fat grafts is crucial for successful outcomes and minimizing resorption.
Purpose of the Study:
- To identify a subpopulation of adipose cells in liposuction aspirate with maximal viability.
- To enhance the survival rate of transplanted fat grafts.
Main Methods:
- Liposuction samples were obtained and centrifuged at 50g for varying intervals.
- Adipose tissue layers (superficial, middle, deep) were analyzed after collagenase degradation.
- Cell viability was assessed using trypan blue staining and cell counting.
Main Results:
- The deepest layer of centrifuged adipose tissue showed a significantly higher concentration of viable adipocytes (250% more than the top layer, 140% more than the middle layer).
- Centrifugation beyond 2 minutes did not yield further increases in viable cell yield.
- A 2-minute centrifugation effectively stratified adipocytes based on viability.
Conclusions:
- Centrifugation of liposuction aspirate for 2 minutes at 50g is an effective method to isolate a layer enriched in viable adipocytes.
- Utilizing the deepest layer of centrifuged fat for transplantation can potentially improve fat graft survival and reduce resorption.