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p16 gene methylation in colorectal cancers associated with Duke's staging
1Department of Cell Biology, Shanghai Second Medical University, 280 Chongqing South Road, Shanghai 200025, China. yijing@shsmu.edu.cn
Aim:
To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.
Methods:
Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.
Results:
p16 methylation was detected in 42% of the tumors.Dukes'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%).
Conclusion:
p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.