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Updated: Aug 19, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
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Effect of angiotensin II on protein phosphorylation in PC12 cell line

B Zieliński1, I Berdowska, E Seweryn

  • 1Department of Medical Biochemistry, Medical University, Wrocław, Poland.

Folia Histochemica Et Cytobiologica
|February 1, 2002
PubMed

Insights

Angiotensin II, via AT2 receptors, dephosphorylates proteins in PC12W cells. Cellular redox mechanisms may control this protein phosphorylation signaling pathway.

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Two angiotensin II receptor subtypes, AT1 and AT2, are known.
  • PC12W pheochromocytoma cells exclusively express AT2 receptors, potentially acting via G proteins or independently.
  • Understanding the dynamic changes in protein phosphorylation in response to angiotensin II and redox agents is crucial.

Purpose of the Study:

  • To investigate the dynamic changes in the phosphorylation pattern of PC12W cells.
  • To explore the effects of angiotensin II and redox agents on protein phosphorylation.
  • To elucidate the signaling pathways involved in AT2 receptor activation.

Main Methods:

  • PC12W cells were treated with angiotensin II and subsequently with redox agents.
  • Western-Blotting with antiphosphotyrosine and anti-ERK2 antibodies was employed.
  • Phosphotyrosine phosphatases and kinases activity assays were performed.

Main Results:

  • Angiotensin II, acting through AT2 receptors, induced dephosphorylation of proteins (60-150 kD) in PC12W cells.
  • The observed phosphorylation pattern suggests a potential similarity to the leukocyte CD45 receptor pathway.
  • Hydrogen peroxide (H2O2) treatment significantly decreased phosphotyrosine phosphatases activity.

Conclusions:

  • AT2 receptor activation leads to dephosphorylation of specific proteins in PC12W cells.
  • Cellular redox mechanisms may play a regulatory role in signal transduction pathways involving protein phosphorylation.
  • The findings suggest a link between AT2 receptor signaling and cellular redox state.

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