Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemoglobin equilibrium analysis by the multiangle laser light-scattering method.

Takamasa Yamaguchi1, Kazuhiko Adachi

  • 1Division of Hematology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Biochemical and Biophysical Research Communications
|February 1, 2002
PubMed
Summary

Multiangle light-scattering (MALS) analysis characterized protein-protein interactions in hemoglobin (Hb) and its subunits. This method accurately determined molecular weights and dissociation constants for Hb A, Hb F, and individual globin chains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure-based prediction of muscarinic M1/M3 receptor antagonist activity: Curated dataset and scaffold-aware evaluation.

The Journal of pharmacology and experimental therapeutics·2026
Same author

Cluster-Validated Graph Neural Network for P-gp Substrate Prediction from Public Data.

Journal of chemical information and modeling·2026
Same author

Structure-Based Prediction of QT Prolongation Risk Using Graph Neural Networks: An Integrative Approach Combining In Vitro hERG Assays and Pharmacovigilance Data.

Clinical pharmacology and therapeutics·2026
Same author

Meroterpenoids with BACE1-inhibitory activity from the fruiting bodies of Suillus bovinus and Boletinus cavipes.

Journal of natural medicines·2024
Same author

Photoreaction products of extract from the fruiting bodies of Polyozellus multiplex.

Journal of natural medicines·2024
Same author

A Scientific Proposal for Surgical Decision-Making in Occult Intertrochanteric Fractures Based on Finite Element Analysis.

Cureus·2023

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Biophysics

Background:

  • Hemoglobin (Hb) exists as tetrameric structures, with complex subunit equilibria influencing its function.
  • Understanding the dissociation and re-association of Hb subunits is crucial for comprehending its physiological behavior.

Purpose of the Study:

  • To evaluate dimer-tetramer and monomer-dimer-tetramer equilibria of tetrameric hemoglobins and their single chains.
  • To assess the utility of microbatch multiangle light-scattering (MALS) for characterizing protein-protein interactions in multi-subunit proteins.

Main Methods:

  • Microbatch multiangle light-scattering (MALS) analysis was employed.
  • Molecular weights and concentration-dependent equilibria of human Hb A and Hb F (CO form) were determined.

Related Experiment Videos

  • Dissociation constants for Hb A, Hb F, and individual globin chains (alpha, beta, gamma) were calculated.

    • Main Results:

    • Human Hb A and Hb F (CO form) exhibited concentration-dependent molecular weights.
    • Dissociation constants to dimers were determined for Hb A (2.58 x 10^-6 M) and Hb F (0.66 x 10^-6 M).
    • Dissociation constants for alpha-chain dimers to monomers (14 x 10^-6 M), beta-chain tetramers to monomers (25 x 10^-17 M), and gamma-chain tetramers to dimers (6.86 x 10^-6 M) were established.

    Conclusions:

    • The MALS analysis system is effective for determining molecular weights of proteins.
    • MALS can successfully characterize protein-protein interactions, including subunit equilibria in multi-subunit proteins like hemoglobin.