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Updated: Oct 2, 2026
Depletion and Reconstitution of Macrophages in Mice
Published on: August 1, 2012
Priming and activation of mouse macrophages by trehalose 6,6'-dicorynomycolate vesicles from Corynebacterium
Mohamed Chami1, Karine Andréau, Anne Lemassu
1Laboratoire des Biomembranes, Institute de Biophysique et Biochimie Moléculaire et cellulaire, Centre de La Recherche Scientifique, Orsay, France.
Abstract:
Vesicles consisting of pure trehalose dicorynomycolate (TDCM), the corynebacterial analog of the most studied mycobacterial glycolipid 'cord factor', were isolated from Corynebacterium glutamicum cells by mild detergent treatment; these induced in vivo a macrophage priming similar to that obtained with mycobacterial-derived trehalose dimycolate. In vitro, both TDCM and bacterial lipopolysaccharide (LPS) induced in macrophages the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), endotoxin tolerance, and were primed for an enhanced secondary NO response to LPS. Interferon-gamma pretreatment did not influence the LPS-induced TNF-alpha response, but considerably increased the TDCM-induced response.
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