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Updated: Aug 14, 2026

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Transport-associated phosphorylation of 2-deoxy-D-glucose in Saccharomyces fragilis
Abstract:
2-Deoxy-D-glucose transport and metabolism was studied in Saccharomyces fragilis. Inside the cells four phosphorylated and three non-phosphorylated derivatives were found and identified. Accumulation of phosphorylated 2-deoxyglucose derivatives was balanced by a concomitant decrease of cellular ATP, orthophosphate and polyphosphates. The free sugar was concentrated against a concentration gradient, contradiciting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation. Theoretical considerations and analysis of the effects of iodoacetate showed that an intracellular hexokinase activity was not involved in 2-deoxyglucose phosphorylation, although this sugar is a good substrate for the enzyme in in vitro experiments.
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