Rapid determination of caffeine in tea leaves
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Updated: Oct 2, 2026
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
Hideki Horie1, Atsushi Nesumi, Tomomi Ujihara
1National Institute of Vegetable and Tea Science, Shizuoka, Japan. horie@affrc.go.jp
Abstract:
For the purpose of efficient screening of low-caffeine tea shoots, a method for the rapid determination of caffeine was developed using high-performance liquid chromatography. Polyvinylpolypyrrolidone was packed in a pre-column and used to remove polyphenols from tea extracts on-line. The concentrations of caffeine extracted from powdered tea leaves at 50 degrees C during 1 day could be analyzed in 2-5 min intervals. The pre-column and the analytical column could be used for the analysis of more than 2000 samples.
