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Updated: Jul 12, 2026

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

Published on: July 17, 2014

Surface charge of Plasmodium falciparum merozoites as revealed by atomic force microscopy with surface potential

M Akaki¹, E Nagayasu, Y Nakano

¹Research Institute of Science and Technology, Tokai University, Isehara, Kanagawa, Japan.

Parasitology Research

|February 2, 2002

Summary

This summary is machine-generated.

Surface charges of Plasmodium falciparum merozoites and erythrocytes were analyzed. The apical merozoite end is positive, while erythrocytes are negative, suggesting a role in erythrocyte invasion.

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Area of Science:

Cell biology
Parasitology
Biophysics

Background:

Plasmodium falciparum causes malaria, a significant global health burden.
Understanding merozoite invasion mechanisms is crucial for developing antimalarial strategies.

Purpose of the Study:

To investigate the surface electric charges of Plasmodium falciparum merozoites and erythrocytes.
To determine the role of surface charge in the erythrocyte invasion process.

Main Methods:

Atomic force microscopy with surface potential spectroscopy was employed.
Transmission electron microscopy was used to visualize particle interactions with cell surfaces.

Main Results:

The apical end of P. falciparum merozoites exhibited a positive surface charge.
The entire surface of erythrocytes displayed a negative charge.
Negatively charged nanogold particles bound to the merozoite apical end, while positively charged ferritin bound to erythrocytes.

Conclusions:

Surface charge distribution plays a critical role in P. falciparum merozoite invasion of erythrocytes.
The positive apical charge on merozoites may facilitate interaction with the negatively charged erythrocyte surface.